Cell
PYB1

Part:BBa_K4231021:Design

Designed by: Shi Han   Group: iGEM22_USTC   (2022-09-30)


Y.lipolytica strain po1f with borneol metabolic pathway establishing in peroxisome


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7589
    Illegal EcoRI site found at 13976
    Illegal EcoRI site found at 20177
    Illegal EcoRI site found at 20227
    Illegal EcoRI site found at 21189
    Illegal XbaI site found at 19664
    Illegal SpeI site found at 15549
    Illegal PstI site found at 634
    Illegal PstI site found at 3745
    Illegal PstI site found at 6126
    Illegal PstI site found at 7256
    Illegal PstI site found at 12579
    Illegal PstI site found at 15366
    Illegal PstI site found at 16152
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7589
    Illegal EcoRI site found at 13976
    Illegal EcoRI site found at 20177
    Illegal EcoRI site found at 20227
    Illegal EcoRI site found at 21189
    Illegal NheI site found at 5898
    Illegal SpeI site found at 15549
    Illegal PstI site found at 634
    Illegal PstI site found at 3745
    Illegal PstI site found at 6126
    Illegal PstI site found at 7256
    Illegal PstI site found at 12579
    Illegal PstI site found at 15366
    Illegal PstI site found at 16152
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7589
    Illegal EcoRI site found at 13976
    Illegal EcoRI site found at 20177
    Illegal EcoRI site found at 20227
    Illegal EcoRI site found at 21189
    Illegal BglII site found at 2741
    Illegal BglII site found at 5983
    Illegal BglII site found at 11502
    Illegal BglII site found at 14893
    Illegal BglII site found at 15025
    Illegal BglII site found at 22458
    Illegal BamHI site found at 255
    Illegal BamHI site found at 6238
    Illegal BamHI site found at 6337
    Illegal BamHI site found at 6877
    Illegal BamHI site found at 12200
    Illegal BamHI site found at 13366
    Illegal BamHI site found at 16528
    Illegal XhoI site found at 1391
    Illegal XhoI site found at 2737
    Illegal XhoI site found at 2779
    Illegal XhoI site found at 4247
    Illegal XhoI site found at 4280
    Illegal XhoI site found at 8292
    Illegal XhoI site found at 9126
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7589
    Illegal EcoRI site found at 13976
    Illegal EcoRI site found at 20177
    Illegal EcoRI site found at 20227
    Illegal EcoRI site found at 21189
    Illegal XbaI site found at 19664
    Illegal SpeI site found at 15549
    Illegal PstI site found at 634
    Illegal PstI site found at 3745
    Illegal PstI site found at 6126
    Illegal PstI site found at 7256
    Illegal PstI site found at 12579
    Illegal PstI site found at 15366
    Illegal PstI site found at 16152
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7589
    Illegal EcoRI site found at 13976
    Illegal EcoRI site found at 20177
    Illegal EcoRI site found at 20227
    Illegal EcoRI site found at 21189
    Illegal XbaI site found at 19664
    Illegal SpeI site found at 15549
    Illegal PstI site found at 634
    Illegal PstI site found at 3745
    Illegal PstI site found at 6126
    Illegal PstI site found at 7256
    Illegal PstI site found at 12579
    Illegal PstI site found at 15366
    Illegal PstI site found at 16152
    Illegal NgoMIV site found at 8
    Illegal NgoMIV site found at 889
    Illegal NgoMIV site found at 1295
    Illegal NgoMIV site found at 3187
    Illegal NgoMIV site found at 4435
    Illegal NgoMIV site found at 5902
    Illegal NgoMIV site found at 6630
    Illegal AgeI site found at 3673
    Illegal AgeI site found at 4538
    Illegal AgeI site found at 7607
    Illegal AgeI site found at 8804
    Illegal AgeI site found at 8891
    Illegal AgeI site found at 9417
    Illegal AgeI site found at 13534
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1415
    Illegal BsaI site found at 2140
    Illegal BsaI site found at 13255
    Illegal BsaI.rc site found at 7650
    Illegal BsaI.rc site found at 8312
    Illegal BsaI.rc site found at 8660
    Illegal BsaI.rc site found at 12974
    Illegal BsaI.rc site found at 13324
    Illegal BsaI.rc site found at 16047
    Illegal BsaI.rc site found at 17953
    Illegal SapI site found at 1248
    Illegal SapI site found at 19260


Design Notes

The strain must be preserved in proper way.


Source

Y.lipolytica strain po1f was given from our PI. All the three plasmids were cloning by infusion cloning, restriction enzyme and T4 DNA ligase.

References